Carmela Oliva, 27, went into labour and delivered the babies -- boys Paolo and Maurizio, and girls Francesca Pia, Angelica, Annachiara and Serena -- in Benevento, near Naples, on Sunday.
They were born 13 weeks premature in the 27th week of pregnancy and are all healthy.
The six babies weighed between 1 lb 5.5 oz and 1 lb 12.2 oz.
Father Pino Mele said he hopes the sextuplets' grandparents will be able to help look after the children, who are their first grandchildren.
Doctors said the multiple birth probably was due to fertility treatment taken by the woman, and not due to in vitro fertilisation.
Italian law prohibits more than three embryos to be transferred into the womb.
The births mark the first case of sextuplets in Italy since 1997.
Last May a British mother gave birth to sextuplets in five minutes after conceiving naturally and without the aid of IVF.
Nuala Conway and her husband Austin defied overwhelming odds to welcome Britain's first sextuplets for a quarter of a century.
But in July, after only 60 days of life, Kerrie Mae Conway, the youngest of the six, died in hospital with her parents Austin and Nuala at her bedside.
The devastated couple, both 26, were called at 5pm on Monday night to say that Kerrie had suddenly become unwell and that her condition was rapidly deteriorating.
The Conways, from Dunamore, Northern Ireland, had put their faith in God and defied doctors' advice to abort some of the babies at 14 weeks to minimise the risks of a multiple pregnancy.
Mrs Conway said in June: 'But we knew without discussion what we both wanted. These babies are a wonderful gift from God.'
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